NCSTN antibody validation - actin antibody in HEK293 knockout line after PNGase treatment

This is the original TIF image of an antibody blot stored in a scaleable vector graphic file. The tif has a dotted line approximately outlining the location of cropping for the paper figure.
The protein source was from HEK293 cells with a CRISPR/Cas9 knockout of nicastrin and wildtype HEK293 cells from the ATCC. One lane has PNGase treatment. The bands are from nicastrin antibody #AB6276.