Mutations in RPS3 affect cleavage efficiency independent of stall sequence.

Northern analysis of 5’ fragments accumulation from ski2Δ strains, with and without mutations in RPS3, carrying different stalling reporters. PGK1 contains a PGK1 mRNA without any additional sequence; SL contains a stem loop at position 1040 of PGK1; (CGA)₁₂, (AAA)₁₂, and (UUU)₁₂ indicate the corresponding codons were inserted at position 950 of PGK1. Corresponding ethidium-bromide stained agarose gel is shown (bottom panel).