Mutation in the <i>sin</i> locus affects <i>C</i>. <i>difficile</i> flagellar synthesis.

<p><b>(A)</b> Heat map showing the lower expression of flagellar and motility-related genes in the R2091::<i>sinRR’</i> mutant compared to the parent. Color intensity in each cell represents corresponding Log<sub>2</sub> expression values in the color scale bar. <b>(B)</b> Transmission electron micrographs of negatively stained <i>C</i>. <i>difficile</i> cells. White arrows point to flagella. <b>(C)</b> Motility of R20291, <i>sinRR’</i> mutant and the <i>sinRR’</i> mutant complemented strains in BHIS with 0.3% agar. The <i>sigD</i> mutant and the <i>sinRR’</i> mutant expressing <i>sigD</i> from an inducible promoter were included in this analysis. The swim diameters (mm) was measured every 24 h for a total of 120 h is shown and the data shown are means ± standard errors of three biological replicates. The experiments were repeated at least three times independently (*, <i>p</i>≤0.05 by a two-tailed Student's <i>t</i>-test).</p>