am508101m_si_001.pdf (419.41 kB)
Multifunctional Polyurethane Sponge for Polymerase Chain Reaction Enhancement
journal contribution
posted on 2015-03-04, 00:00 authored by Seunghwan Seok, Sujeong Shin, Tae Jae Lee, Jae-Min Jeong, MinHo Yang, Do Hyun Kim, Jung Youn Park, Seok Jae Lee, Bong Gill Choi, Kyoung G. LeeSelective
filtering of target biomaterials from impurities is an
important task in DNA amplification through polymerase chain reaction
(PCR) enhancement and gene identification to save endangered animals
and marine species. Conventional gene extraction methods require complicated
steps, skilled persons, and expensive chemicals and instruments to
improve DNA amplification. Herein, we proposed an alternative method
for overcoming such challenges by imparting secondary functionality
using commercially available polyurethane (PU) sponges and cost-effective
fabrication approaches through polydopamine and polysiloxane coatings.
The porous, highly flexible, and chemically modified superhydrophilic
and superhydrophobic PU sponges allow large surface areas and mechanically
stable frames for effective extraction of genomic DNA through selective
filtering of fish tissues and oils. Furthermore, these chemically
modified PU sponges allow separation of genes and improvement of PCR
for DNA amplification for the identification of fish species. The
combination of a simple fabrication method and functionalized PU sponges
could be a useful platform for PCR enhancement and gene-based identification
of species for practical applications.