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Mucosal healing was accelerated by fish oil supplementation.

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posted on 2017-08-11, 17:39 authored by Yunna Lee, Jieun Choo, Su Jin Kim, Gwangbeom Heo, Charalabos Pothoulakis, Yong-Hak Kim, Eunok Im

Mice were fed 2% DSS for 10 days followed by tap water for 5 additional days. During the last 5 days, the mice were supplemented daily with fish oil (FO) or sunflower oil (SO) via oral gavage. (A, B & E) Changes of body weight (A) and diarrhea (B) were monitored daily for a 15-day period. Results are mean ± SEM (n = 8 per group). *P<0.05, **P<0.01, and ***P<0.001. * indicates significance compared with SO group. (C, D) Representative images and histological score of H&E stained colon at D7+W2 and D7+W4 were presented. *P<0.05, * indicates significance compared with SO group at each time point. (E) Colon length was measured at D10+W2 and D10+W4. *P<0.05 * indicates significance compared with SO group at each time point. (F) FACS analysis was conducted to quantitatively determine the percentage of infiltrated neutrophils. PE anti-mouse Ly-6G/Ly-6C antibody was used for staining mouse neutrophils. Three representative histograms of SO and FO groups at D10+W2 were shown. ***P<0.001. * indicates significance compared with SO group. (G) Representative images of colon stained with CD3 and F4/80 antibodies indicate the recruitment of macrophages (green) and T cells (green), respectively. DAPI (blue) was used to indicate nucleus.

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