Movie S1 from Substrate protein dependence of GroEL–GroES interaction cycle revealed by high-speed atomic force microscopy imaging
mediaposted on 23.04.2018 by Daisuke Noshiro, Toshio Ando
Media is any form of research output that is recorded and played. This is most commonly video, but can be audio or 3D representations.
HS-AFM movie of GroEL−GroES interaction during steady-state ATPase reaction cycle in the presence of DM-MBP. D490C GroEL biotinylated at Cys490 was immobilized on a surface of tamavidin 2-LPI 2D crystals in a side-on orientation, while GroES and denature DM-MBP were free in the bulk solution. The images were acquired at 4.35 fps for an area of 64 × 80 nm2. Z-scale, 14.5 nm.