MovieS3.avi

Huh7 exposed to the laser light for indicated time periods with supplementation of 5 mM NAC (1 h pre-treatment with NAC followed by 50 min laser irradiation). Cell viability was detected by the fluorescent live/dead cell assay kit (Thermo Fisher Scientific). After loading the cells with calcein-AM (green) and etidium homodimer (red) images were acquired by confocal microscopy. Labelled cells were exposed to the laser light. Subsequently images were captured using Bio-Rad MRC-1024 laser scanning confocal microscope (Bio-Rad, Cambridge, MA). ImageJ software (NIH) was used for image processing.