Mosaic ancestry, inter-species gene flow and genetic diversity of composite enterotoxigenic Staphylococcus epidermidis pathogenicity islands.
2019-05-22T16:07:02Z (GMT) by
<p>The only known elements encoding enterotoxins in coagulase-negative staphylococci (CoNS) are composite <i>Staphylococcus epidermidis</i> pathogenicity islands (SePIs), including SePI and SeCI regions. We investigated 1545 <i>Staphylococcus</i> spp. genomes using whole genome MLST, and queried them for genes of staphylococcal enterotoxin family and for 29 ORFs identified in prototype SePI from <i>S. epidermidis </i>FRI909. Enterotoxin-encoding genes were identified in 97% of <i>S. aureus</i> genomes, in one <i>S. argenteus</i> genome and in 9 <i>S. epidermidis </i>genomes.<b> </b>All enterotoxigenic <i>S. epidermidis</i> strains carried composite SePI, encoding <i>sec</i> and <i>sel</i> enterotoxin genes, and were assigned to a discrete wgMLST cluster also containing genomes with incomplete islands located in the same region as complete SePI in enterotoxigenic strains. <i>S. epidermidis</i> strains without SeCI and SePI genes, and strains with complete SeCI and no SePI genes, were identified but no strains were found to carry only SePI and not SeCI genes. The systematic differences between SePI and SeCI regions imply a lineage-specific pattern of inheritance and support independent acquisition of the two elements in <i>S. epidermidis.</i> We provided evidence of reticulate evolution of mobile elements that contain elements with different putative ancestry, including composite SePI that contains genes found in other CoNS (SeCI), as well as in <i>S. aureus</i> (SePI-like elements). We conclude that SePI-associated elements present in non-enterotoxigenic <i>S. epidermidis</i> represent a scaffold associated with acquisition of virulence-associated genes. Gene exchange between <i>S. aureus</i> and <i>S. epidermidis </i>may promote emergence of new pathogenic <i>S. epidermidis</i> clones.</p>