Modulation of intercellular signaling pathways that regulate skeletal muscle function in AP-injected HSA-Fv2E-PERK Tg mice.

(A) Representative immunoblots of phosphorylated Akt, total Akt, phosphorylated S6K, total S6K, phosphorylated 4EBP-1, total 4EBP-1, and GAPDH at 8 h after vehicle or AP injections in gastrocnemius muscles of wild-type (WT) and HSA-Fv2E-PERK Tg mice; vehicle or AP (0.1-mg/kg BW) were intraperitoneally injected once a day for 7 days. Right graphs show the band intensity from images (n = 4–5). (B) RT-qPCR analyses of mRNAs encoding regulators of skeletal muscle function at 8 h after vehicle or AP injection in the gastrocnemius muscles of WT and HSA-Fv2E-PERK Tg mice; vehicle or AP (0.1 mg/kg BW) were intraperitoneally injected once a day for 7 days (n = 5). Differences among AP-treated HSA-Fv2E-PERK Tg mice, vehicle-treated HSA-Fv2E-PERK Tg mice, and AP-treated WT mice were considered significant at *p < 0.05 or **p < 0.01.