MoEnd3 is involved in secretion of effectors Avr-Pia and AvrPiz-t.

(A) Pathogenicity of Guy11 and ΔMoend3 was assayed on rice LTH, LTH-Pib and LTH-Pi9. (B) Effector AVR-Pia gene was expressed in Guy11, ΔMoend3, and ΔMoend3/MST7^S212DT216E. Pathogenicity of these strains was assayed on rice LTH and LTH-Pia. (C) Effector AVRPiz-t gene was expressed in Guy11, ΔMoend3, and ΔMoend3/MST7^S212DT216E. Pathogenicity of these strains was assayed on rice LTH and LTH-Piz-t. (D) The bar chart shows quantification of the virulent-type lesions per 5 cm length of leaf. Error bars represent SD. Asterisk represent significant difference and NS represent no significant difference. (E) Images of BICs in the rice sheath cells infected by strains expressing Avr-Pia:GFP. Merged images show DIC and GFP channel. White arrows indicate the BICs. The percentage ± SD of the types of BIC showed were recorded from three independent experiments. In each experiment, 40 BICs were observed for each strain at 24 hpi. Bar = 10 μm. (F) Images of BICs in the rice sheath cells infected by strains expressing AvrPiz-t:GFP. Merged images show DIC and GFP channel. White arrows indicate the BICs. The percentage ± SD of the types of BIC showed was recorded from three independent experiments. In each experiment, 40 BICs were observed for each strain at 24 hpi.