Microtubules polymerized in D2O-Super long tubule

2011-12-30T10:47:43Z (GMT) by Andy Maloney Pranav Rathi
You will see a super long tubule passing through the circle. It takes pretty sharp turns and does not split. I could not track the tubule for long enough, so I do not know what happens next, may be it splits. But in this video it takes successfully pretty sharp turns. Long tubules are slow so may be due to that they do not split as often as short fast ones. Fast ones have more momentum build up, which can produce more force at very quick shark turns. At this point I do not know the threshold force for a tubule to hold-on. We can do an experiment with magnetic bead attached to the head and modulate the strength of the field. As the field is modulated suddenly it will impinge a force on the bead which will cause the tubule to make sudden changes in the direction. So for particular length and speed we can explore this threshold limit. Or we can pull the two ends with optical tweezers, at different loading rates. Also how this changes under different osmotic pressures and heavy water concentration.