Metformin inhibits grade 1 endometrial cancer cell proliferation, survival, migration, and induces apoptosis.

<p><b>A</b>. Sulforhodamine B (SRB) assay measured Ishikawa cell proliferation with increasing concentrations of metformin after 24h or 48h, in high-glucose media. <b>B</b>. Proportion of Ishikawa cells surviving (compared to control) after treatment with 10 mM or 20 mM of metformin. <b>C</b>. Cell migration assay, with Ishikawa cells subjected to control, 10 mM, or 20 mM metformin for 24h. Control (0h) is also shown. <b>D</b>. Quantification of % wound closure; the 10 mM and 20 mM treatment groups were each significantly different than control (p<0.05; noted with asterisk [*]). <b>E</b>. Western blot of apoptosis-related protein expression in Ishikawa cells after treatment with control, 10 mM, or 20 mM for 48h.</p>