Mean error in the center position of the particles measured using: <i>C-Sym</i>, <i>CoM</i>, <i>XCorr QI</i> and <i>GFit</i> algorithms for synthetic fluorescent images using different particle radius (presented in terms of the standard deviation of Gaussian distribution) and noise levels.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0175015#pone.0175015.s003" target="_blank">S3 Fig</a> show examples of the images used to generate these data.</p>