Mass spectrometry of 6His-SipB and endogenous SipB purified from S. Typhimurium.

MALDI-TOF mass spectrometry analysis of intact purified SipB. On the upper left of each graph is indicated the version of the purified SipB protein that has been analyzed by MALDI-TOF mass spectrometry and the organism from which it was purified: Stm for S. Typhimurium. A. The protein 6His-SipB was produced in the S. Typhimurium ΔiacP genetic background from production plasmids co-expressing iacP or not (S2B Fig for production plasmids); B. The intact native SipB was purified from S. Typhimurium WT and ΔiacP (strains JV1 and JV52) using an immunosorbent.