Markhacanasin C, cycloartane triterpenoid from the leaves of Markhamia stipulata var. canaense V.S. Dang

Ngo, Trong Nghia; Vo, Cong Thin; Pham, Nguyen Kim Tuyen; Minh Phan, Nhat; Dat Bui, Trong; Ngo, Quoc Luan; , Van Son Dang; Tran, Cong Luan; Tri Mai, Dinh; Phat Nguyen, Tan

doi:10.6084/m9.figshare.5914777.v1

gnpl_a_1440227_sm7029.pdf (937.77 kB)

Markhacanasin C, cycloartane triterpenoid from the leaves of Markhamia stipulata var. canaense V.S. Dang

journal contribution

posted on 2018-02-22, 12:33 authored by Trong Nghia Ngo, Cong Thin Vo, Nguyen Kim Tuyen Pham, Nhat Minh Phan, Trong Dat Bui, Quoc Luan Ngo, Van Son Dang, Cong Luan Tran, Dinh Tri Mai, Tan Phat Nguyen

One new cycloartane triterpenoid, named markhacanasin C (1), together with three known triterpenoids, oleanolic acid (2), ursolic acid (3) and 6β,19α-dihydroxyursolic acid (4) were isolated by various chromatographic methods from the most cytotoxic fraction of the ethyl acetate extract of Markhamia stipulata var. canaense V.S. Dang leaves. Among them, 4 was reported for the first time from the genus Markhamia, while 2 and 3 were found for the first time from this species. Their structures were elucidated by IR, UV, HR-ESI-MS and NMR experiments. The cytotoxicity of isolated compounds (3 and 4) against three human cancer cell lines (HeLa, HepG2, and MCF-7) were evaluated. At the concentration of 100 μg/mL, 3 exhibited significant cytotoxic activity (86.36 ± 3.69%).