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Manipulating Cellular Activities Using an Ultrasound–Chemical Hybrid Tool
journal contribution
posted on 2017-09-25, 00:00 authored by Ching-Hsiang Fan, Yao-Shen Huang, Wei-En Huang, Albert Alexander Lee, Sheng-Yang Ho, Yu-Lin Kao, Cuei-Ling Wang, Yen-Ling Lian, Tasuku Ueno, Tsung-Shing Andrew Wang, Chih-Kuang Yeh, Yu-Chun LinWe
developed an ultrasound–chemical hybrid tool to precisely
manipulate cellular activities. A focused ultrasound coupled with
gas-filled microbubbles was used to rapidly trigger the influx of
membrane-impermeable chemical dimerizers into living cells to regulate
protein dimerization and location without inducing noticeable toxicity.
With this system, we demonstrated the successful modulation of phospholipid
metabolism triggered by a short pulse of ultrasound exposure. Our
technique offers a powerful and versatile tool for using ultrasound
to spatiotemporally manipulate the cellular physiology in living cells.