MP Transwell migration assay.

(A) Representative images from five randomly selected 10x fields of MPs with/without blebbistatin are shown. (B) Data are from representative experiment out of three performed and denote mean±SEM of migrated cells/field; Statistical analysis were performed using One-way ANOVA, Bonferroni post-test; ** P < 0.01; *** P < 0.001. (C) Zoom of cells located at the lower surface of the Transwell pore, showing fan-shaped lamellipodia containing blebs (marked with asterisks) at the leading edge. (D) Higher magnifications of muscle precursors are shown. (E) Quantification of the percentage of cells with blebs in migrating cells; Statistical analysis were performed using Mann-Whitney test; * P < 0.05.