MOESM6 of Ubiquitin C-terminal hydrolase isozyme L1 is associated with shelterin complex at interstitial telomeric sites

Additional file 6. UCHL1 may interact with RAP1 as part of a nuclear scaffold complex. Nuclear scaffold lysate from DSP-treated DU 145 cells in RIPA buffer was incubated with an anti-UCHL1 antibody or control IgG. The immunoprecipitate (IP), and equal volumes of lysate (Input) and immunodepleted (ID) fractions were analyzed by immunoblotting with UCHL1 and RAP1 antibodies. Mouse/rabbit Rockland TrueBlot secondary antibodies were used.