12934_2016_508_MOESM5_ESM.tif (469.57 kB)
MOESM5 of Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei
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posted on 2016-06-10, 05:00 authored by Christopher Landowski, Eero Mustalahti, Ramon Wahl, Laurence Croute, Dhinakaran Sivasiddarthan, Ann Westerholm-Parvinen, Benjamin Sommer, Christian Ostermeier, Bernhard Helk, Juhani Saarinen, Markku SaloheimoAdditional file 5: Figure S4. Immunoblot detecting IFNα-2b produced by 9-protease deletion strains. Transformants from amp1 (#1), slp7 (#4, 13), amp2 (#5-7), and sep1 (#12) deletion strains were grown in duplicate in 24 well plates in TrMM with 8.6 g/L diammonium citrate, 5.4 g/L NaSO4, 100 mM PIPPS, 20 g/L spent grain extract, 40 g/L lactose at pH 5.5, shaking at 28 °C. The supernatant from day 6 was diluted so that 0.5 µl could be loaded per well. The M577 strain is the parental control (#8). The full length IFNα-2b runs around 17 kDa and the carrier bound material runs at 75 kDa.
