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MOESM3 of Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

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posted on 2018-01-17, 05:00 authored by Ajaya Biswal, Melani Atmodjo, Sivakumar Pattathil, Robert Amos, Xiaohan Yang, Kim Winkeler, Cassandra Collins, Sushree Mohanty, David Ryno, Li Tan, Ivana Gelineo-Albersheim, Kimberly Hunt, Robert Sykes, Geoffrey Turner, Angela Ziebell, Mark Davis, Stephen Decker, Michael Hahn, Debra Mohnen
Additional file 3. Plant height and diameter of three-month-old P. deltoides wild-type (WT), vector control and PtGAUT12.1-OE lines. Values are mean ± SE, n = 25 for WT, n = 10–15 for vector control (V. Control-1-8) and PtGAUT12.1-OE lines (AB29.1–AB29.13). Transgenic values that are significantly different from WT and vector control lines are in bold and denoted with one star (P < 0.05) or two stars (P < 0.001), as determined by one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test using Statistica 5.0.

Funding

The generation of the CCRC series of plant cell wall glycan-directed monoclonal antibodies used in this work was supported by the US National Science Foundation Plant Genome Program (DBI-0421683 and IOS-0923992). The research was funded by The BioEnergy Science Center (BESC) Grant DE-PS02-06ER64304, and partially by the Department of Energy Center Grant DE-FG02-93ER20097 and by the Center for Bioenergy Innovation. The BioEnergy Science Center and the Center for Bioenergy Innovation are U.S. Department of Energy Bioenergy Research Centers supported by the Office of Biological and Environmental Research in the DOE Office of Science.

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