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MOESM3 of User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae

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posted on 2018-12-05, 05:00 authored by Pierre-Louis Lucas, Rodolphe Dumontier, Corinne Loutelier-Bourhis, Alain Mareck, Carlos Afonso, Patrice Lerouge, Narimane Mati-Baouche, Muriel Bardor
Additional file 3. Multistage tandem mass spectrometry analyses of the structure of the LLO-released oligosaccharide isolated from P. tricornutum. ESI-MSn spectra with n = 3 (panel A) and n = 4 (panel B) of permethylated Hex11HexNAc2 derivative (m/z 1413.5 corresponding to [M + 2Na]2+ precursor ion) isolated from P. tricornutum. On each panel, the ion selected for the fragmentation analysis is shown with a diamond and its fragmentation pattern is proposed according to Prien et al. [40]. Black square: N-acetylglucosamine; grey circle: mannose, black circle: glucose. The fragment ions are labelled according to the nomenclature of Domon and Costello [41]. Note that loss of 204 u correspond to the elimination of a 1,2-; 1,3-; 1,4- or 1,6-linked hexose residue while loss of 218 u correspond to the elimination of a terminal hexose residue. Therefore, the successive losses of two 218 u (m/z 1057.5 → m/z 839.4 → m/z 621.3) indicate a di-antenna ion m/z 1057.5 while the successive losses of 204 u (m/z 1057.5 → m/z 853.4 → m/z 649.3) indicate a linear ion m/z 1057.5.

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Institut Universitaire de France

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