40657_2018_117_MOESM3_ESM.docx (435.95 kB)
MOESM3 of Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology
journal contribution
posted on 2018-07-14, 05:00 authored by Ségolène Humann-Guilleminot, Pierre Blévin, Antonin Azou-Barré, Agathe Yacoumas, Geir Gabrielsen, Olivier Chastel, Fabrice HelfensteinAdditional file 3. We checked the minimum number of sperm cells per ejaculate required for accurate estimates of ejaculate mean and standard deviation using a resampling procedure. We randomly sampled 1 to 15 sperm cells per ejaculate without replacement and computed the associated mean (Fig. S1) and standard deviation (Fig. S2). A visual assessment of the plots reveals that a minimum of 10 sperm cells per ejaculate allows accurate estimates of mean and SD.