13068_2018_1220_MOESM3_ESM.pdf (48.58 kB)
MOESM3 of Optimizing the composition of a synthetic cellulosome complex for the hydrolysis of softwood pulp: identification of the enzymatic core functions and biochemical complex characterization
journal contribution
posted on 2018-08-09, 05:00 authored by Benedikt Leis, Claudia Held, Björn Andreeßen, Wolfgang Liebl, Sigrid Graubner, Louis-Philipp Schulte, Wolfgang Schwarz, Vladimir ZverlovAdditional file 3. Effect of inhibitors on the cellulosomalcomplexes. The glucose concentration was measured using the Glu-HK determination kit (Megazyme). The residual complex activity was assessed using 0.5 mL standard reaction mixture containing 0.25% (w/v) of the substrate for one to 2 days of incubation at 60 °C. The initial activity at time point 0 corresponds to 100% relative activity.