12934_2015_382_MOESM3_ESM.pdf (148.36 kB)
MOESM3 of Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds
journal contribution
posted on 2015-12-01, 05:00 authored by CĂŠsar Aguilar, NoemĂ Flores, Fernando Riveros-McKay, Diana Sahonero-Canavesi, Susy Carmona, Otto Geiger, Adelfo Escalante, Francisco BolĂvarAdditional file 3: Figure S1. RT-qPCR values of central metabolism and regulatory genes. Relative mRNA concentrations of central metabolism and regulatory genes of PB12 and PB13 strains, grown in glucose as the sole carbon source were determined by RT-qPCR. The PB12 values have been previously reported [2, 6, 7] and are presented here for discussion and comparison purposes. Data in this Figure are reported as relative expression levels of the parental strain JM101. The mRNA level of each gene in the parental strain was used as control to normalize the data, assigning it the value of one (see Materials and Methods).