MOESM3 of A Pseudomonas putida efflux pump acts on short-chain alcohols

Additional file 3: Figure S3. TtgABC expression does not increase growth of P. putida DOT-T1E in n-butanol. Growth of P. putida DOT-T1E without plasmid (WT), without induction (ttgABC - ), and with induction (ttgABC + ) in 0% (a) and 0.5% (b) n-butanol in 15 mL conical tubes containing 3 mL medium. Similar to the results obtained from the plate reader (cf. Fig. 3), growth of the wild-type strain is slightly faster with and without n-butanol, and TtgABC expression does not increase growth in n-butanol.