MOESM2 of New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica

Additional file 2. Influence of dilution rate on the induction of hybrid EYK1 promoter pHU4EYK300 in continuous culture by erythritol. FL1-A/FSC-A cytograms corresponding to the chemostat of JMY6380 (pHU4EYK300) on YNB-erythritol medium (1% erythritol). The horizontal line at 4x103 FU represents the limit between induced cells (quadrant Q1-UR of the cytogram) and non-induced cells (quadrant Q1-LR of the cytogram). Cytograms are representative of two independent cultures, and are the result of the analysis of 40,000 cells. a Cytogram of the equilibrium cell population cultivated at a dilution rate of D = 0.16 h−1. b Cytogram of the equilibrium cell population cultivated at a dilution rate of D = 0.08−1.