MOESM2 of Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elements

Additional file 2: Figure S2. Related to Fig. 2. a Overlap between SPT16 and TRIM33 peaks in BMDM. b Gene Ontology (GO) annotations of genes nearest to intergenic SPT16/TRIM33 peaks in BMDM. c Scatter plot showing global correlation of SPT16 binding in WT and Trim33−/− BMDM. The Spearman coefficient value is given. d Normalized SPT16 tag count at most enriched (Top10%) intergenic SPT16 peaks that did not colocalized with TRIM33. ns: not significant, Paired t test. e SSRP1 ChIP-qPCR at indicated SPT16/TRIM33 bound regions in WT and Trim33−/− BMDM. Mean ± SEM, n = 3. f PU.1 ChIP-qPCR analysis at indicated SPT16/TRIM33 bound regions in WT and Trim33−/− BMDM. Mean ± SEM, n = 3. g SPT16 ChIP-qPCR in WT and Trim33−/− BMDM (left) and in Trim33−/− immortalized macrophages (IM) and in Trim33−/− IM rescued with exogenous TRIM33 (Trim33−/− + WT TRIM33) (right). h Immunoblotting of FLAG-TRIM33, PU.1 and b-ACTIN in Hela cells transfected with the indicated vectors (left). FLAG-TRIM33 ChIP-qPCR at the reporter vector containing three PU.1 binding sites. Mean ± SEM, n = 2. i PU.1 and TRIM33 occupancy at the Atp1b3/Rnf7 locus in BMDM