MOESM2 of Disruption of the c-Myc/miR-200b-3p/PRDX2 regulatory loop enhances tumor metastasis and chemotherapeutic resistance in colorectal cancer

Additional file 2: Figure S2. c-Myc represses transcriptionally miR-200b-3p expression. (a) Protein expression levels of c-Myc in six CRC cell lines were detected by western blot and quantified by Image J software. The grey value of c-Myc was normalized to that of the corresponding GAPDH. Pearson correlation analysis for miR-200b-3p and c-Myc protein levels in six CRC cell lines. (b) The luciferase activity of PGL3-promoter-miR-200b-3p constructs of wild type and mutant types after transfection of pCDA3.1-c-Myc constructs in 293T cells (*** p < 0.001). (c) miR-200b-3p was detected by qPCR in SW480 and Caco2 cells after transfection of pCDA3.1-c-Myc constructs. The c-Myc protein was measured to assess the transfection efficiency of pCDA3.1-c-Myc constructs by western blot (*** p < 0.001). (d) The transfection efficiency of lentiviral vectors expressing c-Myc and co-expressing c-Myc and miR-200b-3p were measured by western blot and qPCR in SW480 cells. The grey value of c-Myc was normalized to that of the corresponding GAPDH. (*** p < 0.001).