MOESM2 of Application of a delta-6 desaturase with Îą-linolenic acid preference on eicosapentaenoic acid production in Mortierella alpina

Additional file 2: Fig. S2. Construction of binary vectors pBIG2-ura5s-MaFADS6-I (c) and pBIG2-ura5s-MpFADS6 (d). The his 550 promoter fragment was derived from M. alpina ATCC 32222. The trpCt transcription terminator region was derived from Aspergillus nidulans. LB, left border; RB, right border. The binary vector pBIG2-ura5s-IT1 was constructed by Guangfei in our lab [27]. (a) and (b): Cloning of MaFADS6-I and MpFADS6. M: Marker; 1, 2: MaFADS6-I; 3, 4: MpFADS6. (e) and (f): Confirmation of binary vectors pBIG2-ura5s-MaFADS6-I and pBIG2-ura5s-MpFADS6 using MaFADS6-I and MpFADS6 primers. M: Marker; 5, 6: MaFADS6-I; 7: positive control (MaFADS6-I); 8, 9, 10: MpFADS6; 11: positive control (MpFADS6). (g) and (h): Confirmation of binary vectors pBIG2-ura5s-MaFADS6-I and pBIG2-ura5s-MpFADS6 using HisproF/TrpCR primers. M: Marker; 12, 15: negative control (pBIG2-ura5s-ITs); 13, 14: pBIG2-ura5s-MaFADS6-I; 16, 17, 18: pBIG2-ura5s-MpFADS6.