MOESM1 of Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin

Additional file 1. Additional figures including VP cycle, and additional kinetic, Py-GC/MS, SEC and NMR results. Fig. S1. VP catalytic cycle and CI, CII and resting state electronic absorption spectra. Fig. S2. Kinetics of CI reduction by native, acetylated and permethylated softwood and hardwood lignosulfonates: Native VP vs W164S variant. Fig. S3. Lignosulfonate permethylation: Py-GC/MS of softwood lignosulfonate before and after 1 h methylation with methyl iodide. Fig. S4. SEC profiles of softwood and hardwood nonphenolic lignosulfonates treated for 24 h with native VP and its W164S variant and controls without enzyme. Fig. S5. HSQC NMR spectra of acetylated softwood and hardwood lignosulfonates treated for 24 h with native VP and its W164S variant, and control without enzyme. Fig. S6. Kinetics of reduction of LiP CII by native and permethylated softwood and hardwood lignosulfonates. Fig. S7. SEC profiles of softwood and hardwood lignosulfonates treated for 24 h with native LiP and controls without enzyme. Fig. S8. HSQC NMR spectra of native softwood and hardwood lignosulfonates treated for 3 and 24 h with LiP-H8, and the corresponding controls without enzyme. Fig. S9. Difference spectra of peroxidase-treated softwood lignosulfonates minus their controls. Fig. S10. Difference spectra of peroxidase-treated hardwood lignosulfonates minus their controls.