figshare
Browse
40643_2018_229_MOESM1_ESM.docx (1.04 MB)

MOESM1 of Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology

Download (1.04 MB)
journal contribution
posted on 2018-10-16, 05:00 authored by Xudong Zhu, Xiang Shi, Ju Chu, Bangce Ye, Peng Zuo, Yonghong Wang
Additional file 1: Fig. S1. The method of pixels counting. (A) Original photo of the droplets. (B) Adjusted image by adjusting the “threshold” and the pixels were automatically counted. Fig. S2. The growth curve of Bacillus coagulans in shake flask without (black) and with pHrodo (red). The data were obtained from three parallel experiments for each. Fig. S3. Lactic acid assay in microdroplets. (A) Fluorescence imaging of microdroplets containing broth with a range of lactic acid, 8 g/L (I), 11 g/L (II), 12 g/L (III), 14 g/L (IV). (B) The corresponding average gray value derive from the fluorescence intensity of the microdroplets in the images of Fig. S1 A.

Funding

National Science Foundation for Young Scientists of China

History

Usage metrics

    Bioresources and Bioprocessing

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC