MOESM1 of Impaired CD8+ T cell responses upon Toll-like receptor activation in common variable immunodeficiency

Additional file 1. Gating strategy for monofunctional CD4+ and CD8+ T cells. Initially, cells were selected from gates SSC-A and SSC-H (singlets) to exclude doublets; afterwards, we selected lymphocyte cells, live cells and CD3+ T cells, and the subsets CD4+ and CD8+ . Each cytokine was individually defined: IL-17a, IL-22, TNF, IL-10 and IFN-γ.