MOESM1 of Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability
2016-08-04T05:00:00Z (GMT) by
Additional file 1. Non-denaturing PAGE analysis of wild-type and mutant forms of Cel48S complexed with the monovalent scaffoldin ScafÂˇT. Complexes were produced at different molar ratios and applied to non-denaturing polyacrylamide gels. For each gel, lanes 1 to 7 correspond to the respective enzyme/scaffoldin complexes at the following ratios: 0.4, 0.6, 0.8, 1.0, 1.2, 1.4 and 1.6. (Lane 4 signifies the calculated 1:1 ratio). Lane 8 corresponds to the specified enzyme alone at the presumed molar ratio. Red rectangle indicated the determined exact equimolar ratio.