MOESM1 of Engineering Saccharomyces cerevisiae with the deletion of endogenous glucosidases for the production of flavonoid glucosides
2016-08-04T05:00:00Z (GMT) by
Additional file 1. Table S1. Primers used for cloning of S. baicalensis SbGT cDNAs. Table S2. Primers used for cloning of S. cerevisiae EXG1 (GenBank No. M34341) homologous DNA fragments. Table S3. Primers used for cloning of S. cerevisiae SPR1 (GenBank No. NM_001183609) homologous DNA fragments. Table S4. Primers used for cloning of S. cerevisiae YIR007W (GenBank No. NM_001179529) homologous DNA fragments. Table S5. Primers used for cloning of S. cerevisiae PGM2 (GenBank No. NM_001182605) gene. Table S6. Primers used for cloning of S. cerevisiae UGP1 (GenBank No. NM_001179601) gene. Figure S1. SDS-PAGE analysis of recombinant SbGTs purified by affinity chromatography. Figure S2. Effects of various divalent metal ions, pH, temperature on enzyme activity of SbGT34. Figure S3. Activity analysis of whole-cell glycosidase in wild-type S. cerevisiae W303-1b using luteolin 7-O-glucoside as a substrate. Figure S4. Diagrammatic sketch of the knockout of glucosidase genes. Figure S5. A multiple alignment of the amino acid sequences of SbGT30, SbGT34, SbGT56, UBGT, SbUGT and UGT71G1. Figure S6. Schematic diagram of biosynthetic pathway of scutellarein 7-O-glucoside.