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MOESM13 of Genome-wide analysis reveals a role for TDG in estrogen receptor-mediated enhancer RNA transcription and 3-dimensional reorganization

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posted on 2018-01-29, 05:00 authored by Bart Kolendowski, Haider Hassan, Milica Krstic, Majdina Isovic, Gobi Thillainadesan, Ann Chambers, Alan Tuck, Joseph Torchia
Additional file 13. CRISPR-mediated deletion of TDG in MCF7 cells. (A) To eliminate TDG protein from MCF7 cells we used the CRISPR/Cas-9 and pair guided excision to remove a 490 bp region of TDG which contained exon 2 and which also created a frame-shift. (B) Protein levels of TDG in wild-type (WT) cells as well as those edited using CRISPR or siRNA (C: control siRNA or nontargeting CRISPR, TDG: siRNA targeting TDG or CRISPR targeting TDG).

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Canadian Institutes of Health Research

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    Epigenetics & Chromatin

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