MOESM13 of Genome-wide analysis reveals a role for TDG in estrogen receptor-mediated enhancer RNA transcription and 3-dimensional reorganization
2018-01-29T05:00:00Z (GMT) by
Additional file 13. CRISPR-mediated deletion of TDG in MCF7 cells. (A) To eliminate TDG protein from MCF7 cells we used the CRISPR/Cas-9 and pair guided excision to remove a 490Â bp region of TDG which contained exon 2 and which also created a frame-shift. (B) Protein levels of TDG in wild-type (WT) cells as well as those edited using CRISPR or siRNA (C: control siRNA or nontargeting CRISPR, TDG: siRNA targeting TDG or CRISPR targeting TDG).