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MOESM11 of Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

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posted on 2018-01-17, 05:00 authored by Ajaya Biswal, Melani Atmodjo, Sivakumar Pattathil, Robert Amos, Xiaohan Yang, Kim Winkeler, Cassandra Collins, Sushree Mohanty, David Ryno, Li Tan, Ivana Gelineo-Albersheim, Kimberly Hunt, Robert Sykes, Geoffrey Turner, Angela Ziebell, Mark Davis, Stephen Decker, Michael Hahn, Debra Mohnen
Additional file 11. Field design of the multi-year field trial of P. deltoides PtGAUT12.1-OE and control lines. The field was divided into three replicate plots, each contained completely randomized genotype subplots. Each subplot contained two vegetatively propagated clonal trees, planted 3 m × 3 m apart from each other and from other trees in the neighboring subplots. Border trees were also planted surrounding the whole plot to control for shading effects. Grey boxes represent subplots containing transgenics lines with genotypes other than the PtGAUT12.1-OE, vector control, and WT lines.

Funding

The generation of the CCRC series of plant cell wall glycan-directed monoclonal antibodies used in this work was supported by the US National Science Foundation Plant Genome Program (DBI-0421683 and IOS-0923992). The research was funded by The BioEnergy Science Center (BESC) Grant DE-PS02-06ER64304, and partially by the Department of Energy Center Grant DE-FG02-93ER20097 and by the Center for Bioenergy Innovation. The BioEnergy Science Center and the Center for Bioenergy Innovation are U.S. Department of Energy Bioenergy Research Centers supported by the Office of Biological and Environmental Research in the DOE Office of Science.

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    Biotechnology for Biofuels

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    Keywords

    Plant cell wallBiofuelBiomassPectinXylanYield

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