MOESM10 of Failure to detect M. avium subspecies paratuberculosis in Johne’s disease using a proprietary fluorescent in situ hybridization assay

Additional file 10. Comparison of different fluorescent filters (See “Main text”.) “Positive” probe control. For IS 900 MAP Upper left (a) is Texas Red. Upper right (b) is TritC. For Bovine β-actin, (c) is Cy-5 and (d) is “Hope.” Identical section of slide. Although there is a slight difference in intensity, purportedly “positive” signal is seen with both sets of filters, when (a) is compared with (b), as well as when (c) is compared with (d).