am506873g_si_001.pdf (1.55 MB)
M13 Bacteriophage Displaying DOPA on Surfaces: Fabrication of Various Nanostructured Inorganic Materials without Time-Consuming Screening Processes
journal contribution
posted on 2014-11-12, 00:00 authored by Joseph
P. Park, Minjae Do, Hyo-Eon Jin, Seung-Wuk Lee, Haeshin LeeM13 bacteriophage (phage) was engineered
for the use as a versatile
template for preparing various nanostructured materials via genetic
engineering coupled to enzymatic chemical conversions. First, we engineered
the M13 phage to display TyrGluGluGlu (YEEE) on the pVIII coat protein
and then enzymatically converted the Tyr residue to 3,4-dihydroxyl-l-phenylalanine (DOPA). The DOPA-displayed M13 phage could perform
two functions: assembly and nucleation. The engineered phage assembles
various noble metals, metal oxides, and semiconducting nanoparticles
into one-dimensional arrays. Furthermore, the DOPA-displayed phage
triggered the nucleation and growth of gold, silver, platinum, bimetallic
cobalt–platinum, and bimetallic iron–platinum nanowires.
This versatile phage template enables rapid preparation of phage-based
prototype devices by eliminating the screening process, thus reducing
effort and time.