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M117 localizes to viral replication compartments in the nucleus.

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posted on 2018-12-10, 18:52 authored by Eléonore Ostermann, Stefan Loroch, Zhikang Qian, Albert Sickmann, Lüder Wiebusch, Wolfram Brune

(A) Immunofluorescence of 10.1 fibroblasts infected with MCMV-3xFlag-M117 (MOI of 1 TCID50/cell). Cells were fixed 24 hpi and stained with antibodies raised against Flag and the MCMV E1 protein, a marker for viral replication compartments. Nuclei were stained with DRAQ5. (B) Schematic representation of the M117 protein. The positions of the predicted bipartite NLS, the stop codons of the ΔCter and ΔCter2 mutants are shown. (C) Immunofluorescence of 10.1 fibroblasts 24 hours post transfection with plasmids encoding triple Flag-tagged full-length (FL) M117, or truncated M117-ΔCter and M117-ΔCter2 proteins.

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