Localization of CNNM-1 and CNNM-3 at the basolateral membrane of intestinal cells.

<p>(A) Fluorescent images of wild-type L1 larvae with <i>cnnm-1p</i>::<i>GFP</i> or <i>cnnm-3p</i>::<i>GFP</i>. Areas of neurons and intestines showing strong GFP signals are marked. Bar, 30 μm. (B) Quantification of sterile phenotype in wild-type, <i>cnnm-1</i>; <i>cnnm-3</i> mutants, and <i>cnnm-1</i>; <i>cnnm-3</i> mutants expressing either CNNM-1 or CNNM-3 under the control of the promoters for intestinal (<i>ges-1p</i>) or pan-neuronal (<i>aex-3p</i>) markers. More than 50 worms were analyzed for each genotype. (C) Fluorescent images of intestinal cells from wild-type L1 larvae expressing the Venus-fusion proteins of CNNM-1 or CNNM-3 (left). Schematic representation of the intestinal cell plasma membranes are also shown (right). Apical and basolateral membranes are indicated by dotted and solid lines, respectively. Bar, 10 μm.</p>