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Different layers of leukocyte infiltration in nodular lesions six weeks after infection.
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posted on 2016-04-29, 08:16 authored by Miriam Bolz, Nicolas Ruggli, Nicole Borel, Gerd Pluschke, Marie-Thérèse RufHistological section of a nodular lesion six weeks after infection with 1.3 x 106 CFU of M. ulcerans. Ziehl-Neelsen/Methyleneblue (ZN) staining reveals a strong cellular infiltration around a central necrotic core containing AFB stained in pink (layer 1). The necrotic core (layer 1) is surrounded by three more layers (layers 2–4) of leucocytes differing in their cellular appearance. Optical definition of the layers was done as previously described [16].
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