Lipid storage by hiPSC- or hESC-derived hepatocytes in 2D or in 3D on day 13 of differentiation.

(a-b) Lipids detected by ORO (droplets, red-orange). Nuclei stained with hematoxylin (purple). Substantially higher percentage of cells are ORO positive in 3D (b) than in 2D (a) on day 13. (c) Boxplots of percentages of positive cells for ORO for each group. The bottom and top of the box are the first and third quartiles, and the band inside the box is the median. The ends of the whiskers represent the minimum and maximum. Individual data points represented by ○ and ▲ are mild and extreme outliers, respectively. Statistical difference level is indicated by ** for p<0.01 according to the results of the Dunn-Bonferroni posthoc tests performed with the Kruskal-Wallis test (α = 0.05).