Limit of detection and lowest limit of quantitation of the HtrA3 AlphaLISAs.

<p>The limit of detection (LOD) of 10H10C/6G6B and 10H10C/2C4B AlphaLISAs was determined for two matrices (IB and analyte-depleted human serum) from the mean of zero concentration plus three standard deviations in the five assays from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045956#pone-0045956-t002" target="_blank">Table 2</a>. The lowest limit of quantitation (LLOQ) is three times the LOD.</p>