Limit of detection and lowest limit of quantitation of the HtrA3 AlphaLISAs.

The limit of detection (LOD) of 10H10C/6G6B and 10H10C/2C4B AlphaLISAs was determined for two matrices (IB and analyte-depleted human serum) from the mean of zero concentration plus three standard deviations in the five assays from Table 2. The lowest limit of quantitation (LLOQ) is three times the LOD.