Lack of Nox1 catalytic activity does not affect lung inflammatory protein levels or viral titers.
Supernatant harvested from whole lung homogenate was tested for cytokine and chemokine protein levels by Bioplex assay (A-G) and ELISA (H). Supernatant MPO protein levels were determined by ELISA (I). IAV titers were determined by EID50 assay (J). Assay limits of detection depicted by dashed horizontal line. Results in A-I represent mean values ± S.D. All samples run in duplicate; average of two wells used to generate final data. Result in J plotted as individual mice with horizontal bar indicating mean. Data compiled from two independent experiments, n = 7–8 mice per group.