LC-ESI-MS/MS profiling of phenolics in the leaves of <i>Eleutherococcus senticosus</i> cultivated in the West Europe and anti-hyaluronidase and anti-acetylcholinestarase activities

<p>Neither secondary metabolites of the spring leaves nor the autumn leaves of <i>Eleutherococcus senticosus</i> species cultivated in Poland, or the bioactivity are known. The richest in polyphenols was the autumn leaves (171.1 mg/g DE), while in flavonoids the spring leaves (107.9 mg/g DE). Using LC-ESI-MS/MS, protocatechuic acid has been identified as the most abundant compound in the spring and autumn leaves (200 and 70 μg/g DE, respectively). Amongst flavonoids, naringenin 7-<i>O</i>-glucoside occurred in the largest amount (20 and 10 mg/g DE in the spring and autumn leaves, respectively). The autumn leaves inhibited Hyal the strongest (74.3%), comparing to the spring leaves (33%). A weak inhibition was found towards AChE (0.64 and 5.8% for the autumn and spring leaves, respectively). To our best knowledge, no information was available on the phytochemical composition and activity of the leaves of <i>E. senticosus</i> cultivated in Poland.</p>