Knockdown of endogenous psyllid mRNAs by dsRNA feeding on artificial diets after 120 h.

The dsRNAs were added to the artificial diets at concentrations of 200, 500 and 1000 ng.μL-1, and total RNAs were isolated from live psyllids after 120 h of feeding. GFP dsRNA-fed D. citri served as controls for each experiment. (A) Accumulation of cathepsin D mRNAs in whole psyllids fed cathepsin D dsRNAs at 200, 500 and 1000 ng.μL-1. (B) Accumulation of chitin synthase mRNAs in whole psyllids fed chitin synthase dsRNAs at 200, 500 and 1000 ng.μL-1. (A) Accumulation of inhibitor of apoptosis mRNAs in whole psyllids fed inhibitor of apoptosis dsRNAs at 200, 500 and 1000 ng.μL-1. Significant differences in relative expression were evaluated between test dsRNAs and GFP dsRNA at the same concentrations. A single asterisk indicates P<0.05, and double asterisks indicate P<0.01.