Knockdown of endogenous psyllid mRNAs by dsRNA feeding on artificial diets after 120 h.

<p>The dsRNAs were added to the artificial diets at concentrations of 200, 500 and 1000 ng.μL<sup>-1</sup>, and total RNAs were isolated from live psyllids after 120 h of feeding. GFP dsRNA-fed <i>D</i>. <i>citri</i> served as controls for each experiment. (A) Accumulation of <i>cathepsin D</i> mRNAs in whole psyllids fed <i>cathepsin D</i> dsRNAs at 200, 500 and 1000 ng.μL<sup>-1</sup>. (B) Accumulation of <i>chitin synthase</i> mRNAs in whole psyllids fed <i>chitin synthase</i> dsRNAs at 200, 500 and 1000 ng.μL<sup>-1</sup>. (A) Accumulation of <i>inhibitor of apoptosis</i> mRNAs in whole psyllids fed <i>inhibitor of apoptosis</i> dsRNAs at 200, 500 and 1000 ng.μL<sup>-1</sup>. Significant differences in relative expression were evaluated between test dsRNAs and GFP dsRNA at the same concentrations. A single asterisk indicates P<0.05, and double asterisks indicate P<0.01.</p>