K562 cellular adhesion to fibronectin and FN-120 is increased in a time-dependent manner in the presence of an RARγ agonist.

(A) Microtiter wells were coated with 5 μg/mL fibronectin or FN-120. K562 cells cultured for 72 hrs in the presence of vehicle (white bars), 1 μM RARα agonist (light gray bars), RARβ agonist (dark gray bars) or RARγ agonist (black bars) were added to wells (9×10⁴ cells/well) in Hepes-Tyrodes buffer containing 1 mM MnCl₂. (B) K562 cells cultured for 24, 48, 72, or 96 hrs in the presence of vehicle (white bars), 1 μM RARα agonist (light gray bars), RARβ agonist (dark gray bars) or RARγ agonist (black bars) were added to wells coated with 5 μg/mL fibronectin, and adhesion assays were repeated. C) K562 cells cultured for 24, 48, 72, or 96 hrs with vehicle (white bars), 1 μM RARα agonist (light gray bars), RARβ agonist (dark gray bars) or RARγ agonist (black bars) were added to wells coated with 5 μg/mL FN-120, and adhesion assays were repeated. Percent Vehicle = Abs_{405nm(treatment)}/Abs_{405nm(vehicle)} X 100. Results are expressed as means ± SD, n = 6, LSD was used to test for differences among groups. Means followed by * or ** are significantly different (P = 0.01).