K562 cellular adhesion to fibronectin and FN-120 is increased in a time-dependent manner in the presence of an RARγ agonist.

<p>(A) Microtiter wells were coated with 5 μg/mL fibronectin or FN-120. K562 cells cultured for 72 hrs in the presence of vehicle (<i>white bars</i>), 1 μM RARα agonist (<i>light gray bars</i>), RARβ agonist (<i>dark gray bars</i>) or RARγ agonist (<i>black bars</i>) were added to wells (9×10<sup>4</sup> cells/well) in Hepes-Tyrodes buffer containing 1 mM MnCl<sub>2</sub>. (B) K562 cells cultured for 24, 48, 72, or 96 hrs in the presence of vehicle (<i>white bars</i>), 1 μM RARα agonist (<i>light gray bars</i>), RARβ agonist (<i>dark gray bars</i>) or RARγ agonist (<i>black bars</i>) were added to wells coated with 5 μg/mL fibronectin, and adhesion assays were repeated. C) K562 cells cultured for 24, 48, 72, or 96 hrs with vehicle (<i>white bars</i>), 1 μM RARα agonist (<i>light gray bars</i>), RARβ agonist (<i>dark gray bars</i>) or RARγ agonist (<i>black bars</i>) were added to wells coated with 5 μg/mL FN-120, and adhesion assays were repeated. Percent Vehicle = Abs<sub>405nm(treatment)</sub>/Abs<sub>405nm(vehicle)</sub> X 100. Results are expressed as means ± SD, n = 6, LSD was used to test for differences among groups. Means followed by * or ** are significantly different (P = 0.01).</p>