Jujuboside B reduced vascular tension depending on CaM kinase II activity, extracellular Ca2+ influx and TRPC channels.

(A) Representative vascular tension trace showed that KN93 significantly inhibited Jujuboside B-induced vasodilation in endothelium-intact vascular rings preconditioned with PE. (B) Dose response curves showed that KN93 significantly inhibited Jujuboside B-induced vasodilation. ** P < 0.01, n = 4. (C) Representative vascular tension trace showed that EGTA significantly inhibited Jujuboside B-induced vasodilation in endothelium-intact vascular rings preconditioned with PE. (D) Dose response curves showed that EGTA significantly inhibited Jujuboside B-induced vasodilation. ** P <0.01, n = 4. (E) Representative vascular tension trace showed that 4-DAMP did not inhibit Jujuboside B-induced vasodilation in endothelium-intact vascular rings preconditioned with PE. (F) Dose response curves showed that 4-DAMP did not inhibit Jujuboside B-induced vasodilation, n = 4. (G) Representative vascular tension trace showed that SKF96365 significantly inhibited Jujuboside B-induced vasodilation in endothelium-intact vascular rings preconditioned with PE. (H) Dose response curves showed that SKF96365 significantly inhibited Jujuboside B-induced vasodilation. * P < 0.05, n = 4.