Jujuboside B increased intracellular Ca<sup>2+</sup> by inducing extracellular Ca<sup>2+</sup> to inflow through TRPC channels.

<p>(A) Representative confocal image showed that Jujuboside B induced a rapid intracellular Ca<sup>2+</sup> elevation in HAECs instantaneously. (B) Fluorescence intensity curves showed that Jujuboside B increased intracellular Ca<sup>2+</sup> concentration in a dose-dependent manner, n = 4. (C) Analysis of intracellular Ca<sup>2+</sup> concentration showed that Jujuboside B increased intracellular Ca<sup>2+</sup> concentration in a dose-dependent manner, n = 4. (D) Representative confocal image showed that EGTA inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase. (E) Fluorescence intensity curves showed that EGTA significantly inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase, n = 4. (F) Analysis of intracellular Ca<sup>2+</sup> concentration showed that EGTA significantly inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase, ** <i>P</i> < 0.01, n = 4. (G) Representative confocal image showed that SKF96365 inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase. (H) Fluorescence intensity curves showed that SKF96365 significantly inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase, n = 4. (I) Analysis of intracellular Ca<sup>2+</sup> concentration showed that SKF96365 significantly inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase, ** <i>P</i> < 0.01, n = 4.</p>