Jujuboside B increased intracellular Ca²⁺ by inducing extracellular Ca²⁺ to inflow through TRPC channels.

(A) Representative confocal image showed that Jujuboside B induced a rapid intracellular Ca²⁺ elevation in HAECs instantaneously. (B) Fluorescence intensity curves showed that Jujuboside B increased intracellular Ca²⁺ concentration in a dose-dependent manner, n = 4. (C) Analysis of intracellular Ca²⁺ concentration showed that Jujuboside B increased intracellular Ca²⁺ concentration in a dose-dependent manner, n = 4. (D) Representative confocal image showed that EGTA inhibited Jujuboside B-induced intracellular Ca²⁺ increase. (E) Fluorescence intensity curves showed that EGTA significantly inhibited Jujuboside B-induced intracellular Ca²⁺ increase, n = 4. (F) Analysis of intracellular Ca²⁺ concentration showed that EGTA significantly inhibited Jujuboside B-induced intracellular Ca²⁺ increase, ** P < 0.01, n = 4. (G) Representative confocal image showed that SKF96365 inhibited Jujuboside B-induced intracellular Ca²⁺ increase. (H) Fluorescence intensity curves showed that SKF96365 significantly inhibited Jujuboside B-induced intracellular Ca²⁺ increase, n = 4. (I) Analysis of intracellular Ca²⁺ concentration showed that SKF96365 significantly inhibited Jujuboside B-induced intracellular Ca²⁺ increase, ** P < 0.01, n = 4.