J4 male H. glycines undergo extensive remodeling.

(A) DIC micrograph of J4 male remodeling from sausage-shaped J3 to vermiform adult. At this stage the male is wrapped within the J3 cuticle. (B) Transverse TEM micrograph of J4 male at approximate location of box in (A). Note that the TEM micrographs are not of the same animal as in (A). TEM demonstrate the various parts of the male during J4 are wrapped within a thick extracellular matrix. (C) High magnification of boxed region in (B) showing the VNC (purple) with synaptic vesicles and adjacent body wall muscle (green). The epidermis (tan) at this time point is much thicker than during the mobile J2 or the adult male stage (see Table 1). Scale bar, 1 μm.